| 质粒名称: | pC-sleeping beauty |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 65487 |
| 存储实验室: | Guillaume Filion |
|
载体骨架基本信息 |
|
| 载体名称: | pCasper |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 8780 bp |
| 载体修饰: | -- |
| 载体类型: | 昆虫表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | sleeping beauty transposase |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pC-sleeping beauty was a gift from Guillaume Filion (Addgene plasmid # 65487 ; http://n2t.net/addgene:65487 ; RRID:Addgene_65487) |
| 参考文献部分: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pC-sleeping beauty 9852 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION HSP-driven SB100X Sleeping Beauty transposase in pCasper backbone..
ACCESSION .
VERSION .
KEYWORDS pC-sleeping beauty
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9852)
TITLE Guillaume Filion Plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9852)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9852
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 42..471
/label=hsp70 poly(A)
/note="polyadenlyation signal from the Drosophila
melanogaster hsp70Ab gene"
CDS complement(564..1586)
/codon_start=1
/product="hyperactive variant of the Sleeping Beauty
transposase (Mates et al., 2009)"
/label=SB100X
/translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
"
primer_bind complement(1596..1615)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(1597..1615)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(1660..2108)
/label=hsp70 promoter
/note="Drosophila melanogaster hsp70Bb promoter"
primer_bind complement(1692..1713)
/label=pCasper-hs
/note="Drosophila Hsp70 promoter, forward primer"
primer_bind complement(2136..2154)
/label=pCasper-F
/note="Drosophila mini-white gene, reverse primer"
CDS join(2614..2685,3080..3353,3428..4082,4144..4459,4680..4811,
4882..5496)
/codon_start=1
/gene="white"
/product="Drosophila white gene eye color pigment"
/label=mini-white
/note="This is a modified version of the white gene lacking
part of the first intron."
/translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
misc_feature 6274..6859
/label=P element 5' end
rep_origin complement(7094..7682)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7174..7193)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7853..8713)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8476..8495
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8714..8818)
/gene="bla"
/label=AmpR promoter
primer_bind 8886..8904
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(8942..8964)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 9064..9083
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
misc_feature 9620..9852
/label=P element 3' end
/note="P element 3' end"
ORIGIN
1 aagcttggct gcaggtcgac ggatccccgt taattcgagc tctcctgacc gtccatcgca
61 ataaaatgag ccattatgag atcgaaaggg tctacgaaaa tccgttcgga aaaaatcaga
121 aaatcatcaa agccgaatat aattaaaatg tattactagc taaagaaatc atcactaata
181 tagaatgtag aatgaaccca tgtatattag atactaattg tatcgtaaga ctttcaaaag
241 tctacaagac attaaatgac aagttgactt taaatttcaa ataaataatt tattttttct
301 ataagcaata acatttttgc taaattaaga cttggtaatt aggtaatact attgttgttc
361 tatggaatat tcgatcgaaa cattcttatc agtctcaaaa acttaaaaca aacttataat
421 ataacccata tgttataacc cattgatgaa caaaaattag actctttggc cttagtcgac
481 ggatccccga caccagacca actggtaatg gtagcgaccg gcgctcagct ggaattaggc
541 cttctagacc gcggttaggg cccctagtat ttggtagcat tgcctttaaa ttgtttaact
601 tgggtcaaac gtttcgggta gccttccaca agcttcccac aataatttgg gtgaattttg
661 gcccattcct cctgacagag ctggtgtaac tgagtcaggt ttgtaggcct ccttgctcgc
721 acacgctttt tcagttctgc ccacaaattt tctataggat tgaggtcagg gctttgtgat
781 ggccactcca ataccttgac tttgttgtcc ttaagccatt ttgccacaac tttggaagta
841 tgcttggggt cattgtcgtg ttggaagacc catttgcgac caagctttaa cttcctgact
901 gatgtcttga gatgttgctt caatatatcc acatactgca ccgcgtccat gatgccatct
961 attttgtgaa gtgcaccagt ccctcctgca gcaaagcacc cccacaacat gatgctgcca
1021 cccccgtgct tcacggttgg gatggtgttc ttcggcttgc aagcctcccc cttcttcctc
1081 caaacataac gatggtcatt atggccaaac agttctattt ttgtttcatc agaccagagg
1141 acatttctcc aaaaagtacg atctttgtcc ccatgtgcag ttgcaaaccg tagtctggct
1201 ttcttatgtc ggttttggag cagtggcttc ttccttgctg agtggccttt caggttatgt
1261 cgatatagga ctcgttttac tgtggatata gatacttttg tacctgtttc ctccagcatc
1321 ttcacaaggt cctttgctgt tgttctggga ttgatttgca cttttcgcac caaagtacgt
1381 tcatctctag gagacagaac gcgtctcctt cctgagcggt atgacggctg cgtggtccca
1441 tggtgtttat acttgcgtac tattgtttgt acagatgaac gtggtaccgc caggcgtttg
1501 gaaattgctc ccaaggatga accagacttg tggaggtcta caattctttt tctgaggtct
1561 tggctgattt cttttgattt tcccatggta ctagtcccta tagtgagtcg tattaattcc
1621 gcggccgcag atctgttaac gaattcccaa ttccctattc agagttctct tcttgtattc
1681 aataattact tcttggcaga tttcagtagt tgcagttgat ttacttggtt gctggttact
1741 tttaattgat tcactttaac ttgcacttta ctgcagattg tttagcttgt tcagctgcgc
1801 ttgtttattt gcttagcttt cgcttagcga cgtgttcact ttgcttgttt gaattgaatt
1861 gtcgctccgt agacgaagcg ctctatttat actccggcgc tcttttcgcg aacattcgag
1921 gcgcgctctc tcgaaccaac gagagcagta tgccgtttac tgtgtgacag agtgagagag
1981 cattagtgca gagagggaga cccaaaaaga aaagagagaa taacgaataa cggccagaga
2041 aatttctcga gttttcttct gccaaacaaa tgacctacca caataaccag tttgttttgg
2101 gattctaggg ggatcgggga tcaattctag tatgtatgta agttaataaa accctttttt
2161 ggagaatgta gatttaaaaa aacatatttt ttttttattt tttactgcac tggatatcat
2221 tgaacttatc tgatcagttt taaatttact tcgatccaag ggtatttgaa gtaccaggtt
2281 ctttcgatta cctctcactc aaaatgacat tccactcaaa gtcagcgctg tttgcctcct
2341 tctctgtcca cagaaatatc gccgtctctt tcgccgctgc gtccgctatc tctttcgcca
2401 ccgtttgtag cgttacctag cgtcaatgtc cgccttcagt tgcactttgt cagcggtttc
2461 gtgacgaagc tccaagcggt ttacgccatc aattaaacac aaagtgctgt gccaaaactc
2521 ctctcgcttc ttatttttgt ttgttttttg agtgattggg gtggtgattg gttttgggtg
2581 ggtaagcagg ggaaagtgtg aaaaatcccg gcaatgggcc aagaggatca ggagctatta
2641 attcgcggag gcagcaaaca cccatctgcc gagcatctga acaatgtgag tagtacatgt
2701 gcatacatct taagttcact tgatctatag gaactgcgat tgcaacatca aattgtctgc
2761 ggcgtgagaa ctgcgaccca caaaaatccc aaaccgcaat cgcacaaaca aatagtgaca
2821 cgaaacagat tattctggta gctgtgctcg ctatataaga caatttttaa gatcatatca
2881 tgatcaagac atctaaaggc attcattttc gactacattc ttttttacaa aaaatataac
2941 aaccagatat tttaagctga tcctagatgc acaaaaaata aataaaagta taaacctact
3001 tcgtaggata cttcgttttg ttcggggtta gatgagcata acgcttgtag ttgatatttg
3061 agatccccta tcattgcagg gtgacagcgg agcggcttcg cagagctgca ttaaccaggg
3121 cttcgggcag gccaaaaact acggcacgct cctgccaccc agtccgccgg aggactccgg
3181 ttcagggagc ggccaactag ccgagaacct cacctatgcc tggcacaata tggacatctt
3241 tggggcggtc aatcagccgg gctccggatg gcggcagctg gtcaaccgga cacgcggact
3301 attctgcaac gagcgacaca taccggcgcc caggaaacat ttgctcaaga acggtgagtt
3361 tctattcgca gtcggctgat ctgtgtgaaa tcttaataaa gggtccaatt accaatttga
3421 aactcagttt gcggcgtggc ctatccgggc gaacttttgg ccgtgatggg cagttccggt
3481 gccggaaaga cgaccctgct gaatgccctt gcctttcgat cgccgcaggg catccaagta
3541 tcgccatccg ggatgcgact gctcaatggc caacctgtgg acgccaagga gatgcaggcc
3601 aggtgcgcct atgtccagca ggatgacctc tttatcggct ccctaacggc cagggaacac
3661 ctgattttcc aggccatggt gcggatgcca cgacatctga cctatcggca gcgagtggcc
3721 cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat gtcagcacac gatcatcggt
3781 gtgcccggca gggtgaaagg tctgtccggc ggagaaagga agcgtctggc attcgcctcc
3841 gaggcactaa ccgatccgcc gcttctgatc tgcgatgagc ccacctccgg actggactca
3901 tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt cgcagaaggg caagaccgtc
3961 atcctgacca ttcatcagcc gtcttccgag ctgtttgagc tctttgacaa gatccttctg
4021 atggccgagg gcagggtagc tttcttgggc actcccagcg aagccgtcga cttcttttcc
4081 tagtgagttc gatgtgttta ttaagggtat ctagcattac attacatctc aactcctatc
4141 cagcgtgggt gcccagtgtc ctaccaacta caatccggcg gacttttacg tacaggtgtt
4201 ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg atcgccaaga tatgcgacaa
4261 ttttgctatt agcaaagtag cccgggatat ggagcagttg ttggccacca aaaatttgga
4321 gaagccactg gagcagccgg agaatgggta cacctacaag gccacctggt tcatgcagtt
4381 ccgggcggtc ctgtggcgat cctggctgtc ggtgctcaag gaaccactcc tcgtaaaagt
4441 gcgacttatt cagacaacgg tgagtggttc cagtggaaac aaatgatata acgcttacaa
4501 ttcttggaaa caaattcgct agattttagt tagaattgcc tgattccaca cccttcttag
4561 tttttttcaa tgagatgtat agtttatagt tttgcagaaa ataaataaat ttcatttaac
4621 tcgcgaacat gttgaagata tgaatattaa tgagatgcga gtaacatttt aatttgcaga
4681 tggttgccat cttgattggc ctcatctttt tgggccaaca actcacgcaa gtgggcgtga
4741 tgaatatcaa cggagccatc ttcctcttcc tgaccaacat gacctttcaa aacgtctttg
4801 ccacgataaa tgtaagtctt gtttagaata catttgcata ttaataattt actaactttc
4861 taatgaatcg attcgattta ggtgttcacc tcagagctgc cagtttttat gagggaggcc
4921 cgaagtcgac tttatcgctg tgacacatac tttctgggca aaacgattgc cgaattaccg
4981 ctttttctca cagtgccact ggtcttcacg gcgattgcct atccgatgat cggactgcgg
5041 gccggagtgc tgcacttctt caactgcctg gcgctggtca ctctggtggc caatgtgtca
5101 acgtccttcg gatatctaat atcctgcgcc agctcctcga cctcgatggc gctgtctgtg
5161 ggtccgccgg ttatcatacc attcctgctc tttggcggct tcttcttgaa ctcgggctcg
5221 gtgccagtat acctcaaatg gttgtcgtac ctctcatggt tccgttacgc caacgagggt
5281 ctgctgatta accaatgggc ggacgtggag ccgggcgaaa ttagctgcac atcgtcgaac
5341 accacgtgcc ccagttcggg caaggtcatc ctggagacgc ttaacttctc cgccgccgat
5401 ctgccgctgg actacgtggg tctggccatt ctcatcgtga gcttccgggt gctcgcatat
5461 ctggctctaa gacttcgggc ccgacgcaag gagtagccga catatatccg aaataactgc
5521 ttgttttttt ttttaccatt attaccatcg tgtttactgt ttattgcccc ctcaaaaagc
5581 taatgtaatt atatttgtgc caataaaaac aagatatgac ctatagaata caagtatttc
5641 cccttcgaac atccccacaa gtagactttg gatttgtctt ctaaccaaaa gacttacaca
5701 cctgcatacc ttacatcaaa aactcgttta tcgctacata aaacaccggg atatattttt
5761 tatatacata cttttcaaat cgcgcgccct cttcataatt cacctccacc acaccacgtt
5821 tcgtagttgc tctttcgctg tctcccaccc gctctccgca acacattcac cttttgttcg
5881 acgaccttgg agcgactgtc gttagttccg cgcgattcgg ttcgctcaaa tggttccgag
5941 tggttcattt cgtctcaata gaaattagta ataaatattt gtatgtacaa tttatttgct
6001 ccaatatatt tgtatatatt tccctcacag ctatatttat tctaatttaa tattatgact
6061 ttttaaggta attttttgtg acctgttcgg agtgattagc gttacaattt gaactgaaag
6121 tgacatccag tgtttgttcc ttgtgtagat gcatctcaaa aaaatggtgg gcataatagt
6181 gttgtttata tatatcaaaa ataacaacta taataataag aatacattta atttagaaaa
6241 tgcttggatt tcactggaac tagaattaat tcggctgctg ctctaaacga cgcatttcgt
6301 actccaaagt acgaattttt tccctcaagc tcttattttc attaaacaat gaacaggacc
6361 taacgcacag tcacgttatt gtttacataa atgatttttt ttactattca aacttactct
6421 gtttgtgtac tcccactggt atagccttct tttatctttt ctggttcagg ctctatcact
6481 ttactaggta cggcatctgc gttgagtcgc ctccttttaa atgtctgacc ttttgcaggt
6541 gcagccttcc actgcgaatc tttaaagtgg gtatcacaaa tttgggagtt ttcaccaagg
6601 ctgcacccaa ggctctgctc ccacaatttt ctcttaatag cacacttcgg cacgtgaatt
6661 aattttactc cagtcacagc tttgcagcaa aatttgcaat atttcatttt tttttattcc
6721 acgtaagggt taatgttttc aaaaaaaaat tcgtccgcac acaacctttc ctctcaacaa
6781 gcaaacgtgc actgaattta agtgtatact tcggtaagct tcggctatcg acgggaccac
6841 cttatgttat ttcatcatgg gccagaccca cgtagtccag cggcagatcg gcggcggaga
6901 agttaagcgt ctccaggatg accttgcccg aactggggca cgtggtgttc gacgatgtgc
6961 agctaatttc gcccggctcc acgtccgccc attggttaat cagcagaccc tcgttggcgt
7021 aacggaacca tgagaggtac gacaaccatt tgaggtatac tggcaccgag cccgagttca
7081 agaagaaggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
7141 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
7201 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
7261 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
7321 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
7381 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
7441 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
7501 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
7561 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
7621 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
7681 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
7741 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
7801 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
7861 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
7921 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
7981 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
8041 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
8101 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
8161 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
8221 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
8281 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
8341 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
8401 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
8461 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
8521 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
8581 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
8641 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
8701 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
8761 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
8821 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
8881 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
8941 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
9001 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
9061 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcacc
9121 gaatcgcgcg gaactaacga cagtcgctcc aaggtcgtcg aacaaaaggt gaatgtgttg
9181 cggagagcgg gtgggagaca gcgaaagagc aactacgaaa cgtggtgtgg tggaggtgaa
9241 ttatgaagag ggcgcgcgat ttgaaaagta tgtatataaa aaatatatcc cggtgtttta
9301 tgtagcgata aacgagtttt tgatgtaagg tatgcaggtg tgtaagtctt ttggttagaa
9361 gacaaatcca aagtctactt gtggggatgt tcgaagggga aatacttgta ttctataggt
9421 catatcttgt ttttattggc acaaatataa ttacattagc tttttgaggg ggcaataaac
9481 agtaaacacg atggtaataa tggtaaaaaa aaaaacaagc agttatttcg gatatatgtc
9541 ggctactcct tgcgtcgggc ccgaagtctt agagccagat atgcgagcac ccggaagctc
9601 acgatgagaa tggccagacc atgatgaaat aacataaggt ggtcccgtcg gcaagagaca
9661 tccacttaac gtatgcttgc aataagtgcg agtgaaagga atagtattct gagtgtcgta
9721 ttgagtctga gtgagacagc gatatgattg ttgattaacc cttagcatgt ccgtggggtt
9781 tgaattaact cataatatta attagacgaa attattttta aagttttatt tttaataatt
9841 tgcgagtacg ca
//
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